![]() The detection of resistance determinants and sequences related to HGT will not only expose the potential for A. johnsonii to easily acquire exogenous DNA from other bacterial species. The data obtained from our genomic analysis strongly suggested the potential and promiscuity of A. The analysis of blaPER-2 genetic context revealed the presence of the previously described context from the Enterobacteriacea family suggesting genetic exchangeConclusions: Genomic analysis of Aj2199 showed evidence of extensive HGT events indicated by the presence of a wide variety of different mobile elements and resistance determinants in Aj2199. Moreover, 46 complete insertion sequences (IS) and truncated IS as well as the presence of three intact prophages were found. The 240 gene families were comprised of 254 genes, of which, more than 164 of these were annotated as hypothetical proteins and 24 were mobile elements. johnsonii strains, while 240 were exclusively found in Aj2199. Genomic comparison identified 1204 gene families in A. A total of 3737 possible open reading frames were predicted using the RAST server. PCR reactions and conjugation assays were also carried out.Results: Assembled contigs totaled 3,803,969 base pairs with an N50 contig size of 70,198 (max length 286,583) and has a G+C content of 41.4 %. ARG-ANNOT, ISfinder, PHAST, PlasmidFinder, Rfam database and the software Infernal, etc., were used to explore the genome. Identification of homologous genes was carried out using the OrthoMCL method using get homologous software. Average nucleotide identity (ANI) was performed. ![]() RAST predictions were confirmed by BLAST (version 2.0). SPAdes assembler version 3.1.0 was used for denovo assembly and RAST server to predict open reading frames. johnsonii Aj2199 was obtained using Illumina MiSeq. ![]() ![]() Here, we present research on the genome of an Aj2199 clinical strain that was co-producing PER-2 and OXA-58. johnsonii isolates are susceptible, strains harboring a variety of β-lactamases have recently been described. johnsonii has been considered an emergent pathogen that can cause human infections like catheter-related bloodstream infections and peritonitis. Background: In recent years, Acinetobacter spp. ![]()
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